LOCUS       KLU92898     4743 bp    DNA             PLN       19-FEB-1998
DEFINITION  Kluyveromyces lactis suppressor protein (SEF1) gene, complete cds.
ACCESSION   U92898
NID         g2104692
VERSION     U92898.1  GI:2104692
KEYWORDS    .
SOURCE      Kluyveromyces lactis.
  ORGANISM  Kluyveromyces lactis
            Eukaryota; Fungi; Ascomycota; Hemiascomycetes; Saccharomycetales;
            Saccharomycetaceae; Kluyveromyces.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Groom,K.R., Heyman,H.C., Steffen,M.C., Hawkins,L. and Martin,N.C.
  TITLE     Kluyveromyces lactis SEF1 and its Saccharomyces cerevisiae
            homologue bypass the unknown essential function, but not the
            mitochondrial RNase P function, of the S. cerevisiae RPM2 gene
  JOURNAL   Yeast 14 (1), 77-87 (1998)
  MEDLINE   98144791
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Groom,K.R., Heyman,H.C., Steffen,M., Hawkins,L. and Martin,N.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-1997) Biochemistry, University of Louisville
            School of Medicine, 319 Abraham Flexner, Louisville, KY 40202, USA
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Kluyveromyces lactis"
                     /db_xref="taxon:28985"
     gene            1464..4679
                     /gene="SEF1"
     CDS             1464..4679
                     /gene="SEF1"
                     /note="suppresses the lethal phenotype of rpm2 deletion
                     strains"
                     /codon_start=1
                     /product="suppressor protein"
                     /protein_id="AAC39353.1"
                     /db_xref="PID:g2104693"
                     /db_xref="GI:2104693"
                     /translation="MFHCHRENIHQGLNCIRNRVIMAMVLTERAKVHLHSHETIMGRL
                     QWLLSRMLRKMGKIVVNISNNSNAVSKGKAVVNAGHRPVTSCTHCRQHKIKCNASEKF
                     PAPCSRCERMGLHCEIDPQFRPKKGSQLQSLRNDVDELKVKIEYLTRNESLCQGSKTK
                     QYRQNLLQAIKSVDFSYRVAGPTQGQVAKNKISVQTYLTNEPQLLQDSQTTTTNPTTS
                     SNSKVVTPTGSDHSPASHNGGSLSSGKPQLLNDSVPANTKDRLPPVLQIALKRLSQQI
                     SSSSPQNSSPTTTGHSPANDLSSSKQHVVATTNAMPLLPSPHANIDEFVLGDVHISIE
                     KATELHNIFVARFLPYFPIMQSNSATELYSQSQLLFWTVILTASLSDPEPTLYNKLSS
                     LIKQLAIETCWIRTPRSTHISQRLLILCNWPLPNQKVLDDSSYRFVGLAKSLSFQLGL
                     HRGKFMTEFTRTQTSLPEAEKWRTRTWLGIFFAEQLWASILGLPPTSQTDYLIEKARL
                     GDDGELPPILRKLICLANFQGKLCNVMGTSVVSPDGLMDARERAGSLAILERELERLD
                     VKLKFQEDAAVEIYFLYIKLMICCFAFLPETPTEDQTKYVTEAYLCATKIITLLTKLL
                     ETQQLIELPIYIRHSATYSALILFKLYLTPLLLDKYVDSARQSIVTVHRLYRNQLTAW
                     AASVENDISRTASVLEKLNFVLVTHPEVFIEEEGIISRMRSHLTGTLFYDLVWCVHEA
                     RRRQMDPNYDAEAAKENKEKWFKNRRKLYPLPFYNQISKEDFETITQTTPGGTTVTTL
                     VPTKSAIKQAKQLAQSQGDQNGPVTHINGIPLSMLDETGSVNIEGLLANANLNLSNEH
                     STVLASSSSTATRLNADNPTTDTNKFNVQTVFTHNIKKSSKSSDTPTNKPKFNSTSSI
                     PTATPTSEQRAAHNTKTSASILTGDPNSLFSMNNSLQANIQLNKTLSADSNGTSNNNI
                     PNSTAPLNTPDTNSFNSLTRSPDAPSYRKHERILQCCKPGYQYEYLIFTIGYPMHLRV
                     YQLRYQLNRLNVTNIPNGNVNSELDDFFLRQSSGWIEGNSR"
BASE COUNT     1483 a   1004 c    889 g   1367 t
ORIGIN      
        1 gttgcagacc attgcttact catgattccg gagatgttat tttatcatta tatacgaaca
       61 atattctaac cacataccta aattccggcc tgcaaacctg cttaacagaa gcactattcc
      121 aactgtgctg ggaacgggac tgaagagtcg agaaacctat ttgtctcaca aagacactca
      181 caaaaataat tgtcctaccc gtttggctgc tacctttatt tagcttttta cccttaagaa
      241 cgccttttct cggacctttg accttcgaga ataatactca cattcatttc aataaagcaa
      301 ttagttttaa attataaagt gatctactcc gcacaaaggt acaataagat gtacaattga
      361 gatacatggc ggaatgtaac agaacgtaaa gtaagggtgc cggtctcttg accatgaacg
      421 ttacaaacgc aaaaaaatga gtcgcatctc ttttcagttt ttaaaatccg aggcgaatct
      481 ttctatataa cacgggctaa atttcaagga aagacaggaa tgtttttagt ctatatatct
      541 aggcagaatg ggaacagcgg caatctacaa cagttagtac agctatctta gcatattgag
      601 tatccaccga ggcgctacca ctaagaatcg tggttgtttc accttatttc tgcccacatc
      661 gctggaactt cttattttct tgcctgccag tcctttgttg cacactgttt tctatataca
      721 gtgatacaat cggtattgat attgaaatcg acattgattg tttggctttt tttatttttt
      781 ttcggattta acttgaagaa tatctctcca gagagaaaca gttggtcagc tgaaagactg
      841 aatcatttat actagtcgat tagactatat accgtgtgtc caagttttgt tgtcagcgct
      901 ttaactgcca tacctttctt ttgtttctgc tttgtgcgag attttaattt tacattaagt
      961 tagtaccaaa tttttggcca aaaaaaaaat taaaattcaa agaaacgatt taaagtagaa
     1021 aacttgggaa agaagaaaaa gctgacgctg ggaatataat ttgaagaggt ttgtttcatc
     1081 actctacagc tgtcattttc attatttttg ttgatttccc ctacagatac tgtttgccaa
     1141 ctgttatttt gatatttaac ttttgttcaa ctaataccct tttcttcctt caagttgatt
     1201 aaaatttcgg ttttggcaca tttgaatgat tagcagtgga tatacttcaa aaacgttcaa
     1261 acaaagctca aacagatcag gaaatattat cggaagcgac tatagataga atttaacaag
     1321 gctcgcactt ttcatcgttt gcaatcgaaa tatcaattct ttcataatgg gtgatccagt
     1381 gtcttcaaag aggaaatcct ctgtgtccga tgctgtttct acaagtaaga ggcaacacca
     1441 gcaggttaaa gtagaagacg tgaatgttcc actgtcatcg cgagaacatt caccagggcc
     1501 tcaattgcat acgcaacaga gttattatgg caatggtact gacggagcga gcgaaagtgc
     1561 acttacacag tcacgaaaca ataatggggc gacttcagtg gttgctatct cggatgctac
     1621 gcaaaatggg gaagatagta gtaaatatca gtaataatag taatgccgtg tcgaaaggta
     1681 aagcagttgt caacgctggt catagaccgg tgacgtcgtg tacgcactgt agacaacaca
     1741 agatcaaatg taacgcttca gagaagtttc ctgcaccttg ttcgagatgt gagagaatgg
     1801 ggttgcactg tgagatcgat ccacagttca gaccgaagaa gggttctcag ctacaaagtt
     1861 tgagaaatga cgtcgatgaa ttgaaagtta agatcgagta tttgaccaga aatgaaagtt
     1921 tgtgccaagg ctctaaaaca aagcaatata gacagaattt gttgcaagct atcaaatctg
     1981 ttgatttttc atacagggta gcagggccga ctcagggtca agtagcgaag aataagattt
     2041 cagtacaaac ctaccttacg aatgaaccac agttactaca agactctcaa accacaacta
     2101 caaatccaac cacgtcctca aattccaaag tggttacacc cacaggatct gatcattctc
     2161 ctgccagtca taatggtggc agtctaagca gcggtaaacc gcagttgttg aatgattcag
     2221 tacctgcaaa tactaaagac cgtctccctc cagttttgca gatcgctctg aaacgcctaa
     2281 gtcaacaaat ttcttccagt tcacctcaga atagtagtcc aacaactacg ggtcattcac
     2341 ctgctaatga tctttcttca agcaaacaac atgttgttgc aacgactaat gcaatgccgt
     2401 tgttgccatc tcctcacgct aatatcgacg agtttgtctt gggtgatgtc cacatttcta
     2461 ttgaaaaagc cactgagtta cacaacattt ttgtcgccag atttttacca tactttccca
     2521 taatgcaatc aaattcggca actgagctat attctcagtc gcagcttcta ttctggacgg
     2581 tgatattaac agcctcatta tcagaccctg aaccaacgtt gtacaataaa ctttcttcac
     2641 taataaaaca actcgccatt gagacttgct ggatcagaac gccaagatca actcacatct
     2701 cacaacgatt gttaatactc tgtaactggc ctttaccaaa ccaaaaggtt ttggatgatt
     2761 cgtcatacag attcgttggg ttagcaaaat ctctatcatt tcaattgggc ttacaccgtg
     2821 gtaagttcat gacagagttt accagaactc aaacatcatt gcccgaggcc gaaaagtgga
     2881 gaacaagaac atggttaggt atatttttcg ctgaacaatt gtgggccagt atattaggtc
     2941 taccaccgac ctcacagacc gactacttga ttgagaaggc aagactaggt gatgacggtg
     3001 agctaccgcc gattttaaga aagctaatct gtctagctaa ctttcaaggt aaactctgca
     3061 atgtaatggg tacaagtgtt gtttctcctg atggcttgat ggatgccagg gagcgtgccg
     3121 gttctctagc tatcttggaa agagagttag aaagacttga cgtaaaattg aagttccaag
     3181 aggacgctgc cgtcgaaatt tactttttat acatcaagtt aatgatctgc tgctttgctt
     3241 tcctcccaga gactccaact gaagatcaga cgaagtatgt cacagaagca tatttatgtg
     3301 caaccaagat aatcacctta ttgacgaagt tacttgaaac tcagcaacta atcgagcttc
     3361 caatttatat cagacacagt gcaacttatt ctgctctcat attattcaaa ctatatctaa
     3421 ctccacttct cttggacaag tatgtggatt ccgcccgtca atctattgtc acggtgcaca
     3481 ggttatacag gaatcaactg acggcttggg ccgccagtgt tgagaatgac atctctcgta
     3541 ctgctagtgt tttggaaaaa ctcaactttg ttttagtcac tcatccggaa gtattcatag
     3601 aagaggaggg tataatttca agaatgagat cacatttgac aggtacttta ttctatgatt
     3661 tggtttggtg cgttcatgag gccagaagaa gacagatgga tccaaactat gatgctgaag
     3721 ctgctaagga aaacaaagaa aaatggttca agaacagaag aaagttatat ccattgccat
     3781 tttataatca aatttctaaa gaggactttg agactattac tcaaactacg cccggtggga
     3841 ctactgttac aacattggtt ccaactaaga gtgctatcaa gcaagctaag cagctagcac
     3901 aatcacaagg agaccaaaat ggcccagtca ctcatattaa tggtattccg ttatctatgc
     3961 ttgacgagac cggtagtgtc aacattgaag gattattggc caatgcaaac ctcaaccttt
     4021 caaatgaaca cagtactgta ttggcttctt cttcttctac tgctactagg ttaaatgcag
     4081 ataacccaac aactgatacc aacaagttca atgttcaaac cgtgttcact cataatatca
     4141 aaaaatcttc aaaaagcagc gatacaccta cgaacaaacc taaattcaat tcaacttcta
     4201 gcattcccac agctacacca acttctgagc aacgtgcagc tcataatacc aaaacttctg
     4261 caagcatttt gactggtgat ccaaactctc ttttctctat gaacaattca ttgcaagcga
     4321 acatccaact caacaagacg ctttcggctg attctaatgg aacttccaat aacaatattc
     4381 ctaattctac ggcacccttg aatacaccag atactaatag ttttaatagt ttgaccagat
     4441 cccctgatgc cccatcttac cggaaacatg aacgtatttt acaatgctgc aaaccagggt
     4501 accaatatga ataccttata ttcacaatcg gctacccaat gcacctcagg gtatatcagc
     4561 tccgctatca gctcaaccgg ctcaatgtca caaatatacc taacgggaac gtcaactcgg
     4621 aacttgatga ttttttccta cgacagagct ccggttggat cgaaggaaac tctagataat
     4681 gatgatttct tggatggttc tagatgagta gaacagtggc tccgaatttt aacaatattc
     4741 gta
//