Laboratories
The format of the lab section of the course is project-oriented; students carry a series of experiments in parallel throughout the semester. Students perform a series of classical enrichments and isolations, starting from environmental samples collected by the themselves. One of these isolations serves as the starting material for a series of modern molecular biology experiments, in which the student purify DNA, amplify the ribosomal DNA by PCR, and have a portion of this gene sequenced. This sequence information is the starting point for the term project, a detailed molecular phylogenetic analysis of the isolated organism.
You need to bring the following material to the second lab (Jan 23-25):
The TAs
Wednesday lab Friday lab through Feb 15th |
Thursday lab Friday lab starting Feb 20th |
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General information
The Labs
Lab schedule
All labs are held in 1522 Gardner Addition from 1:30 - 4:20, on Wednesdays (section 201), Thursdays (section 202) or Fridays (section 203).
| Unknown | Bacillus | Winogradsky | Purple non-sulfurs | Polysaccharide degraders | Yeast/fungi | Plate count anomaly | |
| Jan 9-11 |
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| Jan 16-18 | Introduction, Safety, and Microscopy | ||||||
| Jan 23-25 | innoculate | innoculate | innoculate | innoculate | innoculate | innoculate | |
| Jan 30-Feb 21 | streak | streak | observe | observe | incubate | streak | |
| Feb 6-8 | restreak | restreak | observe | transfer | incubate | restreak | |
| Feb 13-15 | PCR | restreak | observe | streak | incubate | restreak | |
| Feb 20-22 | prep/gel | FINISH | observe | restreak | incubate | FINISH | |
| Feb 27-29 |
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| Mar 5-7 |
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| Mar 12-14 | restreak |
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| Mar 19-21 | LAB OPTIONAL - SPRING HOLIDAY FRIDAY
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| Mar 26-28 | FINISH |
microscopic counts |
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| Apr 2-4 | DISMANTLE |
FINISH |
plate counts |
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| Apr 9-11 | Finish any remaining work - as needed
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| Apr 16-18 |
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| Apr 23-25 |
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