News

This page is for timely information about the course - reminders, dealing with common problems, &c. The date that anything was last posted on this page is given on the course Home page. Please check this page whenever there's anything new.


December 14, 2013  

Scores for the final exam, lab notebooks, and paper have been posted on the Grades page, final grades have been determined and are also posted, and they'll be entered into MyPack shortly.

So, we're done! You can pick up any of your grades material in my office, but drop me a line to make sure I'll be around. Or, you can pick things up when you get back from break.

Thanks for a great semester!


December 1, 2013  

The Final exam has been posted on the Resources page, and I'll have copies to hand out in class tomorrow.


November 18, 2013  

Data from experiment #10 is has been posted on the Resources page.


November 11, 2013  

Data from experiment #9 is has been posted on the Resources page.


November 4, 2013  

Data from experiment #8 is has been posted on the Resources page.


October 28, 2013  

Data from experiment #7 is has been posted on the Resources page.


October 21, 2013  

Data from experiment #6 is has been posted on the Resources page.


October 07, 2013  

Data from experiment #5 is has been posted on the Resources page.


September 30, 2013  

Data from experiment #4 is has been posted on the Resources page.


September 24, 2013  

The midterm exam has been posted on the Resources page, and I'll have copies to hand out in class tomorrow.


September 23, 2013  

Data from experiment #3 is has been posted on the Resources page.


September 22, 2013  

The schedule has been revised - please have a look on the Schedule page. This includes some due dates and the midterm exam.


September 10, 2013  

My take on what we learned from our preliminary experiments:

  1. 200ul is a good volume to load in each well (100 or less is too little, 300 or more is too much)
  2. 5% (1X) NaCl is at the max of the optimal concentration range for P. fluorescens in LB, or even a bit more. 1-2.5% (0.2X-1/2X) might be better.
  3. Too much inoculum leads to "biphasic" (diauxic) growth curves - use 1:100 dilution (2.5ul in 250ul) or less. 1ul per 250ul sample seems good, but you'll need to be careful measuring this accurately.
  4. Neither yeast extract nor tryptone are required for P. fluorescens growth, but best growth is obtained with a mixture of both. As long as there is even a little of both, the ratio is not very important.
  5. 1X LB is in the optimal range for growth of P. fluorescens. (But...it would be interesting to try titrating YE and tryptone while keeping NaCl low, to see if we could get better growth.)

Anything else?


September 09, 2013  

Data from experiment #2 is has been posted on the Resources page.


September 04, 2013  

Data from experiment #1 is has been posted on the Resources page.


August 04, 2013  

Welcome to MB 460!