BCH 768

Comparative analysis of RNA structure


JWB

James W. Brown
Department of Microbiology
NC State University

4558A Thomas Hall
Raleigh, NC 27695-7615

(919) 515-8803 voice
(919) 515-7867 fax
james_brown@ncsu.edu
http://www.mbio.ncsu.edu/JWB/


Lecture
materials
pdf   Slide show (7.2 Mbyte PDF)
pdf   Handouts (0.8 Mbyte PDF)


Readings
pdf     Brown JW and Ellis JC, Comparative analysis of RNA secondary structure: 6S RNA, In Handbook of RNA Biochemistry, Wliley-VCH, Weinhein, 2005 pp 491-512
This is a 'hands-on' description of the initial stages of the comparative analysis of an RNA secondary structure. (11.8Mbyte PDF)

pdf     Michel F and Costa M, Inferring RNA structure by phylogenetic and genetic analysis. In RNA Structure and Function, CSH Laboratory Press, Cold Spring Harbor, NY. 1998 pp 175-202
This is an elderly review of the whole comparative approach, but focued on the intermediate state - the refinement of the secondary structure and the identification of higher-order structure. (2Mbyte PDF)

pdf     Massire C, Jaeger L and Westhof E 1998 Derivation of the three-dimensional architecture of bacterial ribonuclease P RNAs from comparative sequence analysis. J. Mol. Biol. 279:733-793
This is also an elderly paper, but a wonderful example of bringing everything together into a useful three-dimensional model. (3.8Mbyte PDF)

Last updated January 17, 2011 by JWBrown